LOCUS       CO961004                 657 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16a02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:UBC2_CAEEL P35129 UBIQUITIN-CONJUGATING ENZYME
            E2-17 KD, mRNA sequence.
ACCESSION   CO961004
VERSION     CO961004.1  GI:51334188
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 764
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 caagatttgg gccgcgatcc gccagcccaa tgcagtgccg gaccagttgg cgacgacctt
       61 ttccattggc aggcaactat catgggacct cccgagtctc cctaccaagg cggcgttttc
      121 ttcttcccca tccactttcc tactgattac ccgttcaaac cgcccaaagt tgcttttacc
      181 actcgcatct accatccgaa tatcaacagc aacgggagca tttgcttgga tattcttaga
      241 tctcagtggt ctcccgcact gaccatctca aaagttctgc tctcgatctg ctcacttctc
      301 tgtgatccga acccggacga cccattggtg ccagagattg cgcgcatcta caagaccgat
      361 cgcgagagat acaacacact tgcacgggaa tggactcaga agtatgctat gtaacgggca
      421 cggccgggat aaagcgaagg ctagtggtgg atatttgaaa tggcagatgg atgtttggat
      481 ctttttatga cgcggcaaat ttgacaatcc gttttttcat ccctttctcg acttatatta
      541 aataaaaatg aaggagagtc gggttgccgt cgtctttcca actcaaatcc tgtattttcc
      601 tctcggctaa cgtgataaaa aatattgctc gtaaaaaaaa aaaaaaaaaa aaaaaaa
//