LOCUS       CO961002                 720 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15h10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:3O5B_RAT P31210 3-OXO-5-BETA-STEROID
            4-DEHYDROGENASE ;contains element MSR1 repetitive element, mRNA
            sequence.
ACCESSION   CO961002
VERSION     CO961002.1  GI:51334186
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcccatttca gaccattgta caagtggtgc tgcgttttct gacgcagctc ggcctctgcg
       61 tggtccccaa gtccagctcg cctgagaggc agcgccaaaa tttggctctg ttcgactttg
      121 cgctttcgga cgcggaaatg gcccagatgg aggctctgga cacgcgcgct cggatcatcc
      181 acctgaagct gagcgaacac tcgccgctgt tcccctgggg cgagggaaag cccgacttct
      241 gagttgtgtg tgactcggaa cgcccaattg acccattccc acataagctg cctccaatcg
      301 agttccccat ccttatccgg gctgttcctt gttgatgctc tggctgccct atttttagcg
      361 ccgcactaat ccatccactt cgaggtcata tcttatctca acgtacacgt tcatacactt
      421 catattgctt ccttgatcgc ccattttgga tgctagacat tagtttacaa tgttttagtg
      481 cacattttaa aataaaaata gttgtacctc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      541 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      601 aaaaaaaaaa aaaaaaaaac caagccccca aaattttttt ttcccggggg gaaattttgg
      661 aaaattccct cccccggggg ggccccccaa ccggtctttg gggggcccca ttccccccaa
//