LOCUS       CO961001                 510 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15h09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to WP:CE25595 Y73F8A.27, mRNA sequence.
ACCESSION   CO961001
VERSION     CO961001.1  GI:51334185
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 524
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aaaatcgctg actccattgt cgagaatttc acggaacttg tctacttcta ccatcagaaa
       61 aacgggtgac tatgagtacg aggatccaaa gtctgaggac gaagttgtca acattaccta
      121 catcacgaga gacagaacgg agcgaaaagt gcgcggcaaa atattcaggg tgcgtgtgaa
      181 gcgtcgctgg cgtgttgtac ttgtcacgtt tacgtggacg agcagttcta cgacaaattg
      241 cctgctccgg tggaggacga ggaggacatg ctggacatgg ccccgctgct gaagccgaac
      301 tcacggctgt cctgtcagat catcctcaac aaggaactgg agggactggt ggtttcactt
      361 ccaaaaatta cgaggaattt ctatgtcgat ggccacgttc cagagcctca ttgatccttt
      421 ttttttgctc agatttcata tttataaaaa ttaagctaca aaaaaaaaaa aaaaaaaaaa
      481 aaaaaaaaaa aaaaaaaaaa aaaaaccaaa
//