LOCUS       CO960999                 725 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15h07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to contains element TAR1 repetitive element, mRNA
            sequence.
ACCESSION   CO960999
VERSION     CO960999.1  GI:51334183
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 660.
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cactcattct gtttgatttt cgccacacaa aaatgctcta ccggaaaatg atgatcgcgc
       61 cctcggctgc cgtcgtgatg atgtcactcc tggtcagcac aggcctctgc tcggctcttc
      121 tctggcagca gaacgagccg aaagacaagg ccgctcagcc ggcacaggag gcagcagcag
      181 caccgctctt cttcttcccg gcagcaaatg cagcggctct ggagcagttg gccgccgaag
      241 aggcgcaact gattgcggag gcggcggcac aacaggcggc ggccgacgag gagcaagaag
      301 cggagcttgc cgctctgaag ccgaagcggg cgcagacgtt cgtccgtttc ggcaaacggt
      361 cgcagatgga aaatgaaatg gcggctggca tgaagccgaa gagagcgcag acatttgtgc
      421 gcttcgggaa gagggcaccg atgatggagg gcgagaagga cgaagccgcg ctgcagaatg
      481 aaaaacgggc gcagactttc gtccgattcg gcaaactagc gcagacgttc gtgcgctttg
      541 gcaagcgggc tcagaccttc gtgcgcttcg gaaagagagc tgccccgcaa caacaacagg
      601 aggcggagaa ctgatttgct ggctgagggc gagtgtggcg gtcgatctct ccttttcaca
      661 ncaacaacna cactcccaca catccgtttg tctctctgat ccaccataac caccaccatt
      721 cgcat
//