LOCUS       CO960998                 570 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15h06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YV31_CAEEL Q20516 HYPOTHETICAL 6.8 KD PROTEIN
            F47B7.1 IN CHROMOSOME X. [1], mRNA sequence.
ACCESSION   CO960998
VERSION     CO960998.1  GI:51334182
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 571
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gctgtacatg aaactgcttc tgttcgcctc cgtgctcttg ggcaccctcc tttgttccgc
       61 ctacggcgac tcagttgccg ttgagaagga cggctccatc gcttctctcg gcgtcggaga
      121 cgagggcacc gcgttggaga agttcctgac ccgcacgaag cgaagcagcg gcagatggac
      181 cacccagcag atcatcgagt gcatcttctg catattcatc ccgcccgtcg ccgtcctgat
      241 ccacgggggc cacgagtggg tgctgcacct gatcatcaac atcgtgctct ggatcttggg
      301 ctggattccg ggcaccatcc acgccttctg gtactgcttc ttccgttgag cccaatcccc
      361 ctctcttctt caatactcct ctacccccgg ttataagcat tttgcgttcc cttcctgctt
      421 ctagtagtgt gactttttgg ctgttgccca ccaccaaatc catccactca tgaatttctc
      481 aacgaccgat aaatttataa ataaactgat tccatttaaa aaaaaaaaaa aaaaaaaaaa
      541 aaaaaaaaaa aaaaaaaaaa aaaaaccaaa
//