LOCUS       CO960997                 721 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15h05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q95008 Q95008 F25H2.9 PROTEIN. [1], mRNA sequence.
ACCESSION   CO960997
VERSION     CO960997.1  GI:51334181
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 870
            Seq primer: SL1 primer
            High quality sequence stop: 713.
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gagtcataca ttttctccgg aaggacgcct atttcaagtt gagtatgcga ttgagacagt
       61 aaagttgggt gccacaagca tcggtatcca cacccgggat ggaatcttgt tggctgcaga
      121 acgcacatca atgagcaaac tagttgtccc agaatcgatg agcaaaattg ccaatttgga
      181 taagcatctt gtctgtgcct cggctgggct gatcgccgat gctcgcactt gggtggaaca
      241 cgcgcgagtg gaggcacaaa acttttggtt cacctacggg aagaagattc gcgttgaaga
      301 cataacttat aaggtttcca aactggcgct gcactttggc gacgatgact cggagatccg
      361 tctcggccgt ccgttcggcg tgtccatgct gtttgccgga atcgaccaca cgggggcgca
      421 tctcttccat cttgacccgt ccggcaccta cacaaagtgc ctggcaaagg ccatcggggg
      481 cggggcggat ggagccgaac agaccctgca ggagcactgc aaaggcagcg acaagaagat
      541 ggatatggac gaggcaaaga aagtcgccct ggacacccta aagcagctga tggaggagaa
      601 gatcaacgtc aaaaatgtcg aggttgtcct catcaccccg ggcaccgatg aaaatggcga
      661 acagattgga aacatcacat ggatgaccga acaggaactt ggcgaagtga tttcacgcct
      721 a
//