LOCUS       CO960991                 679 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15g08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9VT06 Q9VT06 RPS9 PROTEIN, mRNA sequence.
ACCESSION   CO960991
VERSION     CO960991.1  GI:51334175
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 695
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gcacgtattt tctaaagtct atgacgtgcc tcgtcgtcca ttcgagaaag agcgacttga
       61 tcaggagctc aaactcattg gtgagttcgg tctcagaaac aagcgagagg tgtggcgtgt
      121 caaactgacg ctggccaaga tccgaaaggc ggcccgtgag ctgctgactc tcgaggacaa
      181 ggaccaacga cgtctgttcg aaggcaatgc gctgctccgt cgtctggtcc gagttggcgt
      241 gctgcccgag gacaaaatga agctcgacta cgtgcttggt ctccgcgtcg aggacttctt
      301 ggagcgtcgt ttgcagacac aggtgttcaa actcggactc gccaagtcca tccaccacgc
      361 acgtgtcctc attcgccaaa agcacattcg tgtgcgtcga caggtcgtga acatcccatc
      421 gttcattgtg cgtctggact cgcagaagca catcgacttt tcgaccaagt ccccgtacgc
      481 aggaggacgc cctggccgcg tcaagcgacg cagcatgaag aagggcaccg gcggcggatc
      541 tggaggcgaa gaagaggact aagctccccc agaaagacca atttgagaat gtcgaggtgt
      601 tgatttgttg tttggaacac acgtactatt aaagttgttt tgttaaaaaa aaaaaaaaaa
      661 aaaaaaaaaa aaaaccaaa
//