LOCUS       CO960990                 679 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15g07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O02065 O02065 SIMILAR TO C. ELEGANS FEMALE
            GERMLINE-SPECIFIC TUMOR SUPPRESSOR GLD-1. [1], mRNA sequence.
ACCESSION   CO960990
VERSION     CO960990.1  GI:51334174
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 catctttctc aatctcatct atatgactat ggactatctt gctgaactca tccgtgaaaa
       61 gagcactgtg gcggcatttc ccaatcactt caaacatgtt gatcgcttaa tagacttgga
      121 aattgctcga gttcgcgctc agctcttcca atgcagcttt gacgatgatc ttccacgtct
      181 tcctgagatt gattcgaacg aggacattgt tacgcttcaa gaaaaagttt atgtgccgaa
      241 gaaggatcat ccggagttca atttcgttgg tcgcattctc gggccacgcg gaatgacagc
      301 aaagcaattg gagcacgaga ctggctgtaa gatcatggtt cgtgggcgtg gatcgctgcg
      361 tgacgacaag aaggaagagc tcaaccgtgg aaagccgaat tgggaacatt tggaggacga
      421 cttacacatt ctgattcagt gcgaagatac tggaaagcgt gcacgtgcga aacttgacaa
      481 ggctgttgag aagattaaga aattgctcgt tccatcgcga gatggcattg atgagctcaa
      541 acgaaagcag ctaatggagt tgagcattat caatggaact tatcgcgcat gccccgggca
      601 aaagaactcg attgccattc ctcgtctcac accgctctcc atgttgtcgc ctattcattc
      661 gccgatttcg cctccgcca
//