LOCUS       CO960989                 647 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15g05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q93576 Q93576 F25H2.5 PROTEIN. [1], mRNA sequence.
ACCESSION   CO960989
VERSION     CO960989.1  GI:51334173
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 675
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttttaatagc catttatcga cgcaaatacg cgatgtctac taaaaaccaa gaacgcacat
       61 tcatcgcgat caagcctgat ggtgtccaac gcggtcttgt gagcaagata atcaaacaat
      121 ttgagcagcg tggttataaa ctggtggcta tcaaaatggt caaagcaacg ccccaacact
      181 tggaagtgca ttatgaggat ctgaaggaca aacctttctt cgctggatta gtgaagtaca
      241 tggcttcagg gccagttgtg gcaatggttt gggaaggact cgatgttgtg aaacaaggtc
      301 gagctatgct cggagccaca aatccactgg tgagtgcagc aggaacaatt cgtggaagct
      361 actgtatgga catgggacga aacatctgcc acggatcaga cagtgttcca tcggcagagc
      421 gtgaaattgc acactggttc aagcaggaag aattggttga ctgggagcca gcacagatca
      481 actggatcta cgagtgagga acaatctgga agacgcccat ctgcgataag gaagaaagtc
      541 ccggatacca attcacaaga ccatttgatt tcgaggattt cttattctta ttgtgtcgaa
      601 acttaaagtt gacaatgtta aaatttgtgt tgtgaaaaaa aaaaaaa
//