LOCUS       CO960987                 531 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15f11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:EF1B_BOMMO P29522 ELONGATION FACTOR 1-BETA', mRNA
            sequence.
ACCESSION   CO960987
VERSION     CO960987.1  GI:51334171
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 909
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tggccacaaa aagccggtgg acctccttcg accagtgctg ccaaacaatc ttctccgccc
       61 aagggagcag ccgctgccgc cggcggagat gacgatttcg acctgttcgg cgatgagcac
      121 agcgatgact aacagaagaa gcgaatcacc gaggagcgct tgaaggccta cgcggagaag
      181 aagtccaaga agcccggccc catcgccaag tccaacgtga tctacgatgt cgagccctgg
      241 gacgacactg tggacatcaa ggagatcgag aagaatgtgc gctctattca cacagagggg
      301 cttgtctggg gcacttccaa gatcctgcca gtggcattcg gcgttaagaa gcttcagatt
      361 tgctgctgtg tggaggacga aaaggtctca actgactggc tagaggagca gatcactggc
      421 tttgaggacc tcgtccaatc ggtcgatgtg gtcgccttca acaaagtctg atgagcgatt
      481 gatgagcagc aggagcctat tattgacttt cgcaggcttg gtttgggacc c
//