LOCUS       CO960985                 634 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15f07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to WP:CE25600 Y73F8A.6, mRNA sequence.
ACCESSION   CO960985
VERSION     CO960985.1  GI:51334169
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 658
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcgttcttcg ccgtcgttct actctgcgca ctttgcgtgc ccctggcgca ctcattgtcc
       61 ttgaccaagc gagagagtgg tctggacgcg gaaaaagtgc gcaccgccga gaacaaggcg
      121 cgtctgaacg aggagttcgt cgggatggtg gggcatctcc gcgaggggtg ttttccgcgc
      181 cccgggggca agggctgtcg ctgtgtggag aaggaccccg agggcaggga cgtggaggtg
      241 cggttcggcg ccgatcagga gcagaagtgc cgcgccgctc cgagagtgcg ccgccagacg
      301 cgccgagacc agaacgtgcg ggatccggtc cgcgagcagc aggagcggaa ctacgcggcg
      361 gtgctggacg agctgaagaa caagttcgcg ggactgcgcc agggctgtta cccgcgcccc
      421 aaggggtgcc tgtgcgtagt gggaaaggac gccactggac gcgagatcac cgaacgacgc
      481 atgaaggaca gcgactgcaa gtgtcgcaag gacgagcgca atgcggagtg ccccgcggcg
      541 ggagcgtgaa agaactatcg tattagatga gcaaatgaac ggtccattct gttcggaaag
      601 agattcttca cagaaaaaaa aaaaaaaaaa aaaa
//