LOCUS       CO960983                 550 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15f02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q23683 Q23683 ZK970.7 PROTEIN. [1], mRNA sequence.
ACCESSION   CO960983
VERSION     CO960983.1  GI:51334167
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 614
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ctcgcccagc atggcgaaac cccttcaccg ccgtttctgg agggcgcgtc cgcagagacc
       61 gtgaaatcat tccacgactc gctgtccaac ctgggccact tgactgacca gcagattgac
      121 gacaaagtca aggaatgggt gggccagcag tccgaagacc tcaagaccaa atatgccgca
      181 tttgagatgg agaggcaaaa ggcatcggaa gccggtgagc aggcacacca ggcagcagtg
      241 gccaaattca gcgcagaggc aaaggcggcg gacgacaaga tcagcgcaat tgccaacaat
      301 ccggctttgg cggccaagga caaggccgca cagattcagg ccatcgtgga gggactgccg
      361 cagaatgtgc gcgacgagat ggataaagcc atgggcattg gggggcactg aacaacgagc
      421 aatcgagtgg aaacgaaaaa caccggttga ttcaatgaca ctcgaagctg aattgaatgg
      481 ctttgcacaa atcacagtaa taaacagtag ttccaaaaaa aaaaaaaaaa aaaaaaaaaa
      541 aaaaaaaaaa
//