LOCUS       CO960982                 715 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15e12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:GSHC_CAEEL O02621 PROBABLE GLUTATHIONE PEROXIDASE
            F26E4.12, mRNA sequence.
ACCESSION   CO960982
VERSION     CO960982.1  GI:51334166
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 760
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 agtgcgtcta cgatttttcc gcgaaagaca ttgacggaaa ggaggtgaag ttcgacaagt
       61 accgcggcaa ggtgctgatg gtcgtgaatg tggcgtccca gtgcgggctg accgagagca
      121 actacaacca gctgaaggag actctggaca aatacaagga gaagggactc gccatagccg
      181 cattcccgtg caaccaattc aatggacagg agcccggctg ccctatcgac atcaaggaaa
      241 ctgtgaacgt gaaatacaac tttgagccgg acttgtacga cgaagtcgac gtcaatgggg
      301 acagcgcaca ccctctgtgg aaatatctgc agaaggagaa gggaggcatc cttggctggg
      361 acaaaataaa atggaacttc accaagttct tgatcgaccg caacggaaac gtggttgacc
      421 gttatgcccc gaccacagaa ccgaaaaaga ttcacggcga cattgagaag ctgttggcct
      481 agatgaacga tcattatttg atcgcacttt taatccgaat ttgtgattcc ggtttcccta
      541 agtcctaacg cgactccggc gagcatacct attacatgtg tagaaacgaa ttggacccct
      601 ccatccgtca ccaaacaaca atcgtactct ttgacaccgt agtaaactcc gggtgatcaa
      661 cgaatgattc tccacacgtc tctctgttcg gatttgatat tcatgctggg ctgtt
//