LOCUS       CO960979                 728 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15e09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q22782 Q22782 SIMILAR TO RAS-RELATED PROTEIN. NCBI
            GI: 1125833. [1], mRNA sequence.
ACCESSION   CO960979
VERSION     CO960979.1  GI:51334163
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 802
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcttctgatt ttggatatcc cttgaagaat ttaaattggt atctcctttt tagcaaagcg
       61 ttggcaagac ttcactcgtt acagggttca tgtacgacag ttttgacaat acatatcacg
      121 cgaccattgg gattgatttt ctcaccgaga cgatgtatct tgcggacaga acggttacac
      181 ctcagctttg ggacaccgcc ggacaggaac ggttccattc actcattcca tcctatattc
      241 gcgattctac cgttgccgtg gtcgtttacg acatcacaaa tgcaaattcc ttccaccaaa
      301 cttcgaaatg gattgatgat gtgcgcaccg aacggggcag cgatgtaatt ataatgctcg
      361 ttggcaataa aacagatctg gctgacaaga gacaagtttc tacagaagag ggtgagcaga
      421 aggcaaagga gctgaatgtc atgttcattg agacatcggc caaagcaggc tacaacgtga
      481 aacagttgtt ccgaaggatt gccagtgctc ttcctggaat tgaagtggag aagtcatctg
      541 atgcgaagca agttgtggac attgggccaa tcaagccacg ccagattgtg accgaagaag
      601 gatcgtgctg gtgttaagcg ccgggatgcg gcaaaacaca atccttttaa atgtggacct
      661 ttttgaaatt gatttttacg attaattttg attattaata ttatttatgg acatatgtat
      721 ttgtttgt
//