LOCUS       CO960978                 442 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15e08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O17287 O17287 W10D9.5 PROTEIN. [1], mRNA sequence.
ACCESSION   CO960978
VERSION     CO960978.1  GI:51334162
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 457
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 attgactggg acgcggtgcc cgacgaccag gttgaggaga cgctgctcga gcggctagag
       61 ggcctcacag agatgtttcc gcccggactg cgcagcgcaa ccagctccac cttcggctgg
      121 ggctgctggc tggccaagag ctcgctctcc ctggcgcgca gcactgcgtg gattgtgtcg
      181 accagcgcgt tcatcatgat catgccctac ttcgtggaca aggagctgca agagatggag
      241 aagcaccaga tgaagcagca gcaacagctg ctcctgggac gggcttgagg gaggcggcgg
      301 accagagaaa actttttaat tgattgattg attagtcgtg ggcctggcaa gtttgggggc
      361 acatctcgat tctattgtta ctttttaaat ttgaatttct tttaaaaaaa aaaaaaaaaa
      421 aaaaaaaaaa aaaaaaaaca aa
//