LOCUS       CO960976                 813 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15e03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q17166 Q17166 CUTICULAR COLLAGEN BMCOL-2, mRNA
            sequence.
ACCESSION   CO960976
VERSION     CO960976.1  GI:51334160
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 492.
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 atggaccagc cgagcagcaa ggaggacgcg cggcgcgagg ccgaggcatt gcgcacactg
       61 gccttcttcg gggtgtgcct gtgcacggcg gccatgctcg tcactgtggt gtccgtgcca
      121 ctgtgctacc aatacgtgca acgccaggcc tcccagatgc agtcggagtt ggacttctgc
      181 aggagcaggg cgggcagcat ctggggcgag gtgacgcgca cccagggcat ggcctcggtg
      241 cagggcaaag tacgccacgc gcgccattcc ggcttcctga ctgcctggtc ctcgccgatg
      301 cgcagcggcg agctgaggag gtccgcgccg aggcgccaac gtgacggcta tgggggcgcc
      361 tcggcgggtg tctccagcgc tcctgcgtcg gcgggcggct gctgcggctg tggagtgtcg
      421 cagccgggcc cacccggtcc catgggaccg ccggggccct ccggacagga cggacactca
      481 ggaatggcgg gacgccgggg agaggacgct cggccacgtg cacagccggc cgcctacttt
      541 gtggagccgt gccaacattg cgagaaggcc gaggaccgac ctcctgagtt gcggggaccc
      601 ccggggcctc caggaacctc tgggcggcgc ggtcagcctg gccaaccggg gcatcggggt
      661 ggccgtcgcc cacccggacc ggcgggtccc tcgggccgac ccgggacact acgataactc
      721 ggcctacctg gaaagccggg aaaacttaag gagggccccc cgtgcaaggc cccccggacc
      781 gccgggccac gagggccacc ttgtgcgccg ggc
//