LOCUS       CO960975                 692 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15e02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO960975
VERSION     CO960975.1  GI:51334159
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gcggacgcgt ttctgctgtt ttactcaaac ggcgcattga acaagatggc gacttgactt
       61 ggcatgaagc accttattgc tgcgaagagg gccatgagcc tgcacactgg ctggccgtgg
      121 aggaggcggc actccgattc gagcatgaca atcgagccga gatccaggcg cggatgattg
      181 gacggctagc cgagtgcgga aaggcggcca atttccgcac attcaaaaag attgtggatg
      241 tgtactttga attgctgcaa caccgcttgg accaaggagg agagagtccg caaattccca
      301 gcattctgtg cgtgcccatt ctggctctgg tcgcgttcac ggggttgtat gcgcgaaaat
      361 tggccgaagc ggatgcagaa ctgggagatc gtaacagaga ctttcgcaga gaactctacc
      421 aagtgtccat ctatgggtcg gccgtgctca gcgaaaaact gcgcctattt caatactcat
      481 gggaccattt ctgcggagag acgcgcattc tgctagaacg aatcgcttca cagtatcccc
      541 aatccgcctc ctcccgtctg ttcattggtg tcccaatcgc aatttgtggc cttgctgccg
      601 ctgcctattt cttcatccgt cgcacagcga ttacaacttg agagcgggaa ttcactgata
      661 cctacctata atataatatg taaatcccat gc
//