LOCUS       CO960972                 520 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15d09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to WP:CE21147 T02H6.11 ubiquinol-cytochrome c reductase
            complex subunit, mRNA sequence.
ACCESSION   CO960972
VERSION     CO960972.1  GI:51334156
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 529
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcccaatcgg cgtccagaag ggaatcactt tcaaattcaa gggcaagttc tgggactggg
       61 tgcgccaatt ctgctggacg tacggatggt ccggtcgtca gtatggactt caaatgcacg
      121 accattgttg ggagcctgcg cccgaggtgc gtgaggcact gcgccgcctc cgcctcaagg
      181 acccatacgc gtttgatcaa cgcctccagc ggatgatgtt ggcccagcaa ttggcgaatt
      241 ccaatgagcg tttgccgcgc gaacagtgga ccaaatggga ggacgagacc ttctatctga
      301 agccctatct ggaagaggtc gagcaagaga agctggagcg caaccgcagc agtgggctca
      361 agccgggcta ccaactcaag caccaagtct gaaagcgact ggacgcacca catgtgttag
      421 gcaattcgac catttcggta aatgtgttcc gcctttagat gtacccattt tcggataaag
      481 ttgattaatc aaaaaaaaaa aaaaaaaaaa aaaaaacaaa
//