LOCUS       CO960971                 555 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15d08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q21265 Q21265 SIMILAR TO METALLOPROTEINASE
            INHIBITOR 2 PRECURSORS. [1], mRNA sequence.
ACCESSION   CO960971
VERSION     CO960971.1  GI:51334155
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 713
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tttgtttgcg ttcacaactc ccttgcgtct taaacagaga aacgttcagc acacccctta
       61 gaggggagat atgctcgtgt tagtgttcgc gctgttcccc tgtgccgccg tccacaccac
      121 tggggcgtgc caatgccaaa tggacacgcc caagaacaac tactgcgcat ccgaccgggt
      181 tgcgcacgtg caggtggtga agcgccagga caacgtgcga atgccggccg gcatcgctga
      241 ccgccagacg gaggcgaata cacggcacga gatgaagtac cagcgcatgt tcaagatcat
      301 caaggagatg ccggtgaacg acaagaacca ggtgattttg ccgatcaacg tgtacgcgcc
      361 gatggaggac gcggcgtgcg gcattctgtt ggagccgggc cacaagtacc tgctgtccgg
      421 ccagtacgtg aacggcacca tgctgaccgg gctgtgccgc ctaattctgc tggaggactt
      481 gaaggagtcg cgcaaacacg acatcctgga gtgggccgaa gtgcccgcct agaccaaaga
      541 gcagctggag aagca
//