LOCUS       CO960970                 536 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15d06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:P91889 P91889 FMRFAMIDE, mRNA sequence.
ACCESSION   CO960970
VERSION     CO960970.1  GI:51334154
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 537
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgcccgtcgg caatagccac ccaccaccaa aacatggtcc cgtcagtgca gcagcacctg
       61 atttccgtgc gcccgctgtt gctcctcgcc ttggccacca caatgctcgt gacgctgtcc
      121 agctctgccc tcggcgtcga cgcgatcccc tacaacccgc aacagcagct gcagcgatac
      181 gcgcccatac agagtctgtt cgacgccgag gaggcgttgt tagacgagcc atttgcgcgc
      241 gcggcacgag agcccggtgt gaagtggatg cgattcggga agcgggcccc gcaaggaaag
      301 tggatgcgct tcggcaagcg ggcgcctaac gccggcaagt ggatgcggtt cggtaagagg
      361 acggaggcga atggcgtcga aagaatggaa atggagtgag gcgaggaaag agaaaccaac
      421 cacaagcgtt taacggccag cgaggcggcc aatgagtgtg caacaaacaa tgccactttt
      481 gaaaataaat cgtttccctt tgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aacaaa
//