LOCUS       CO960965                 520 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15d01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO960965
VERSION     CO960965.1  GI:51334149
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 521
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cggacgcttt agggcacgag agcgaacttg gacgagtcga gcggcgccca tctcgactgc
       61 acggcgaatg gcgttcatcg catggaacag cacccccgag gaggtggatg cgcccgccga
      121 agcggaggag ccgaacaaca atggaacaat gcaaactggg agcagcgctg aggaagaagt
      181 gaagagcgcc attcggccaa cagacggaac aacaacaaca gagcaagaag gaaacggacg
      241 aacaacaaaa acaaagcaca gccgcagagt ctttctcgac gcattcggca cccccagcag
      301 agtctttcac aagccattca ttttcggtct ttcatccccg caatccacca tttgttgccc
      361 gcaccccaaa agcccaaaaa cagaactaca aacacacaca aacacaattg tcgcggtcag
      421 cactgtgccc ggaggactgt gaccaaaaca tgccctaaat gtgcgaatga ataaaacgga
      481 ttcaccacaa aaaaaaaaaa aaaaaaaaaa aaaaaacaaa
//