LOCUS       CO960964                 730 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15c12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO960964
VERSION     CO960964.1  GI:51334148
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 772
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cttcgaaagc tgattttgaa atggtttgcg atcaaataat cactgagcag atggactcaa
       61 tgttttcgga agttcgacag aagtgtgctt ccttgcttgc tcaaaccatc gaaaagaagc
      121 gcgccagcct cggatcgcaa caatcggcgg agacaagtga acttcagcaa ggaacggtgc
      181 agacaatcga tggaagcgtt gaccaggtca ttgaattatg cgagaaactt tctgaattgc
      241 ttgcaatttt ggagaatggc gtggattcac ggatgaaagt gaacaataga cttaacgact
      301 tagtctcacg cttcgcgaac gccaacgaga agcataatga aatttacaac aagtccgcca
      361 aatttactct ggatgccatt ctcgacaaga ttgtcaagaa tttggatggt tttgcacgcg
      421 caaacaaaga gatcgattca atcaaacagc tcaaaataga ggcggagagg cgcatgaaca
      481 cttcgaaggt caatgtcgag caaactgcga agagcattga agcgatggat caggaattcg
      541 gtcgactttg cgctcactat gaccaacttt gaaacaaaaa tattgttctc cattaccatt
      601 ggctattgtt taagattaat cagcgatcat ggtatttcgg tcatctcgta ccgattattg
      661 taatcgtcaa taatctatca agtttactgc ggataaaaat atgatttaaa aaaaaaaaaa
      721 aaaaaaaaaa
//