LOCUS       CO960963                 670 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15c11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q93576 Q93576 F25H2.5 PROTEIN. [1], mRNA sequence.
ACCESSION   CO960963
VERSION     CO960963.1  GI:51334147
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 685
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tttatcgacg caaatacgcg atgtctacta aaaaccaaga acgcacattc atcgcgatca
       61 agcctgatgg tgtccaacgc ggtcttgtga gcaagataat caaacaattt gagcagcgtg
      121 gttataaact ggtggctatc aaaatggtca aagcaacgcc ccaacacttg gaagtgcatt
      181 atgaggatct gaaggacaaa cctttcttcg ctggattagt gaagtacatg gcttcagggc
      241 cagttgtggc aatggtttgg gaaggactcg atgttgtgaa acaaggtcga gctatgctcg
      301 gagccacaaa tccactggtg agtgcagcag gaacaattcg tggaagctac tgtatggaca
      361 tgggacgaaa catctgccac ggatcagaca gtgttccatc ggcagagcgt gaaattgcac
      421 actggttcaa gcaggaagaa ttggttgact gggagccagc acagatcaac tggatctacg
      481 agtgaggaac aatctggaag acgcccatct gcgataagga agaaagtccc ggataccaat
      541 tcacaagacc atttgatttc gaggatttct tattcttatt gtgtcgaaac ttaaagttga
      601 caatgttaaa atttgtgttg tgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      661 aaaaaccaaa
//