LOCUS       CO960962                 625 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15c09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O48829 O48829 PUTATIVE GLYCINE-RICH, ZINC-FINGER
            DNA-BINDING PROTEIN, mRNA sequence.
ACCESSION   CO960962
VERSION     CO960962.1  GI:51334146
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gtgagtcgag agttgaggag gcgacgttga gttgagaaga gagacgagaa gaagcgagtt
       61 gagaagatga gctctgggat cgaatgctac cgatgccatg agatgggcca catctcccgc
      121 gagtgtccca actctggtgg tcaaggaggt gggcgtggtg gtggaggacc acggggaggc
      181 ggtggagatg tgaaatgcta caattgtaac ggctatggac atttcgctcg cgattgtcgt
      241 gatggtggtg gagaccgccg tggcgggggc ggtggtggtg gacgaggcga tgaccgcggc
      301 actaagtgct acaactgcgg agggtatggt cactttgcgc gcgaatgccg tgatgggggt
      361 ggagaccgcc gtggaggagg cggacgtgac aaccgtggcg atgatcgccg aggaggcggt
      421 ggaggtggcg gccgtgacgg tggcttcaac tgcggcgaga gcggacactt ctctcgtgaa
      481 tgtcctaagg gtggaggagg aggacgcggt ggcggtggcg gagatcgacc atgctacaac
      541 tgcaacgagt ccggacatct gagccgtgac tgcacgaagg actcgcgcta gagcgcgtgc
      601 cacaaatggc accggtgtgg aaagc
//