LOCUS       CO960960                 703 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15c07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to contains element MSR1 repetitive element, mRNA
            sequence.
ACCESSION   CO960960
VERSION     CO960960.1  GI:51334144
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gtcgtttccc ccgccgcacc agccctactc caacaggatg gacggcaacc caacctacgc
       61 catgtccatg cctgcccacg tctatcccga gacgcactcc cccgtccacg gcaaaccgaa
      121 gcgaacattc ggtgagaagg tgaagatggc cctctacttg gtcgctgtgg cactgctggt
      181 gcttctgctg ctggccatcg tgttctccgc cgtcatgatg ctgttcgacg agaatcgcgc
      241 ctttggacgc gcactccgcg gctggaaggt ctggagaagt ggcggcaacg ggaacgacag
      301 gaacgcgatg cagacgaacg aatgatgaac aggaggactg taaatgggaa agggaccacc
      361 aattcactgt gtgttcaacc ctccaacctc caactccaat gcctcccgca cgtccataat
      421 gtccactctc cactgtcatt gtgctcattc tactatgatt tcctttgact ttttttgcca
      481 tttatacttt aattttattg cctattagta gcagaaataa aaatgataaa taattaaaaa
      541 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      601 aaaaaaaaaa aaaaaaaaag aaaaaaaaaa aaccaattcc ccaaattttt ttctccctgg
      661 ggggaaattt cgaaaaattc ccaccccagg ggcgcccccc caa
//