LOCUS       CO960956                 781 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15b05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9XTD8 Q9XTD8 6R55.1 PROTEIN. [1], mRNA sequence.
ACCESSION   CO960956
VERSION     CO960956.1  GI:51334140
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gctgttcaaa cagcaaactt gttattcaca ctcatttatc tttggtcatt ctacacagat
       61 tatgaattga caaagattta tcggatcctg ccagtgcccg gtctttacta cagcaagttg
      121 gtgtggctaa caatcgtcaa tctctgtctg caacaactct accatgcagt tggtgccatt
      181 ttggccatgt ttgacgttag aaaaacatcc attcttcatc cgtttcactt tgtcgcgact
      241 accctggcat ttccagtttc gatgacagtc gtgttgctgt tctggggact cgtctaccta
      301 gatccgaact tccttgtcaa ggaggagaat gcattccttt tcgaacacaa gttcttcaac
      361 cacagcattc acactttccc actcttagcc atgcttgttg acttctccat ttggaaccac
      421 tctcgtcctt ctcggcgcgg ggctttcatt gccgtggcga tattcaccgc tgcttacctg
      481 attgacattc actatttcta ctacacacac ggcttctggg catacccaat tcttggacaa
      541 ctcgcgccct ggtctcgtgt tttgttcttc gtgttctgct cgattttcat tttctgcact
      601 tttatcgccg gcgacctcgt caatggatgg atccacgcca tgtggcgtca caaaaagagg
      661 gaggggcgaa aggggaaaag aaatgctaaa aaagttgcct aggaatagta aagaaacgca
      721 cattccgaac aattgttaaa tcaaatccca tggacaaatt caacaatggt cggttcgttt
      781 c
//