LOCUS       CO960955                 641 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15b04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q94569 Q94569 SEC-2 PROTEIN, mRNA sequence.
ACCESSION   CO960955
VERSION     CO960955.1  GI:51334139
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 722
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttgttcacga gatggcggca atcaaataca tcttgtgcgc aatcgcgctg cttgcactgt
       61 gccagcccaa caacgcctac tacttcttca gctcctccct gaatgaggca ttcaaaggcg
      121 tgttacccgg tgacgtgtcg gcactgtacg cccaatttga cgcggcagag ttggcgcagg
      181 tgaagttggc cggtgcccag ttggataatg gcggcgacgc tgacgcactg cgcatcctgc
      241 agcagatcaa cgagccgctg cactctcgtg tacttcaggc acgcgacggc atccaacaca
      301 agattgactc tctgcaagtg gaggcgcacg actttgtgga gtcgctgatc gagcaggcca
      361 gagcgttgcg cccaagtggt ggcgcagtgg agcaggcgga caccaagaag acgcggctgg
      421 ccgctgccca catcatcaaa cagttgaatg cgctcagcgc cgaggcgcgt gccaatttgg
      481 gacgcgtgtt ccccgaaatt gtgaccggca tcgagagcac ccaattccag gcgttggcca
      541 atggacagag cgaattgtga gcagcggcag aagaagaagg gaaggggaca gaaaggaaaa
      601 agactcataa agcactgact ttttggatca tgttttgatg c
//