LOCUS       CO960954                 795 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15a12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O44738 O44738 F57B10.5 PROTEIN. [1], mRNA
            sequence.
ACCESSION   CO960954
VERSION     CO960954.1  GI:51334138
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 799
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gggatgagta taaattttgt tccgccgaac aactgctgct gttcgctctg ctctacttgg
       61 aactgtacgc gtatgcttct gagctcacat tcgaactacc tgacaacgcc aatcagtgct
      121 tttacgaaga gatgaagaaa ggcattgact gtacacttga gttccaggtt gtcactggtg
      181 ggcaatatga tgtggatgtg gttttggagg acccaaatgg tcgagttatg tataaggagg
      241 cccggaaaca attggacagt cacccgtaca agaccgaagt tgacggaacc tacaaagtgt
      301 gtttttcgaa cgagttctca accttttcgc ataaagttgt ttacatggac tggcaagtgg
      361 gagctcctga attcactgga ttgcctactc cacgcgttgg aggtggaact tcacagatgg
      421 aacgcagtgt tgagacgata gctgatcgac tcaggattgt ggacgactat caaacacatc
      481 atcgtttgcg cgaagcaact ggccgaaaga gagcagagga tttaaatgaa cgcgtgacct
      541 actggtcctt ggggcaaaca gcggtcgtgg tcataatcgg tattggacag gtgttcttgc
      601 tgagatcttt tttttcggac agaaaaccag ctggatacta atggatgatc aattctattg
      661 gtggataaat tatttgcttg tctactagtt tgtcgcatat tattttttgt caattatctg
      721 caccagttat aaaaaagttt tgttcttgtt ttaaatagag cgatgaaaat tgaaaaagca
      781 taaaaaaaaa aacaa
//