LOCUS       CO960952                 563 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15a09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:FABH_ASCSU P55776 FATTY ACID-BINDING PROTEIN
            HOMOLOG PRECURSOR, mRNA sequence.
ACCESSION   CO960952
VERSION     CO960952.1  GI:51334136
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 588
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tggcactgtg tcttttatta atccaacttt tccgccttac cgctccggcc gtttccaatg
       61 agctgccgga aaagttcctc ggaacctggt ccgtggacca cagtgacgac gactttgacg
      121 cctacctgga ggccaagggc tacaacttct tcgagcgcca actcgcgaag ctggccagtc
      181 tgagcaagac gttcaaaaag aacggagacg gcacctacac cgagacaatg gagagcacct
      241 ccaaggacgt ggagtggccc aacttccgtc tgggcgagca gttccaaggc gaatatttgg
      301 acgacacgca gcacaaaatc ctgatcacgt acagtccgtc cgaagacgca ctcatcgaaa
      361 accaccagga ggtggacacg gacaagaggg aggtgttcaa gtacaccgta agtgcggagg
      421 gctggctggt gatgcagatg gagtacaacg gggtgaagac gaagcgctac tacaccaagg
      481 aatgagccaa cgctatattt taatatttga gtccaataaa catccccaaa tcacaaaaaa
      541 aaaaaaaaaa aaaaaaaaac aaa
//