LOCUS       CO960951                 455 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15a06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:THIH_ORYSA Q42443 THIOREDOXIN H-TYPE, mRNA
            sequence.
ACCESSION   CO960951
VERSION     CO960951.1  GI:51334135
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 464
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gcacgagtgc aaaacggtgg ggggacttcg actcgcagct ggccagggcc ccccgtctgg
       61 tggtcgtgga cttcttcgcc acctggtgcg gcccttgcca gaccatggcg cccgtcttcg
      121 gggccctgtc cgagaagtac gggcagcagg ccctcttcct caaggtggac ggcgactcgg
      181 aggcgggcga tccgctgaca gagcgattcc aagtggaggc cttcccgaca ttcgtcattc
      241 tgctggacgg cgccgagatt ggccgagttg tgggcgctga cgaggaacaa ctggaacaga
      301 gcattcagaa tggcctcaaa cgcctctgaa ctgcgacaaa cgcaagagca ataaaactgc
      361 ggaaacactc acattttgac tgaaaatgtc agtaattctc caggacactt gcaccaaaga
      421 cttttaaaaa aaaaaaaaaa aaaaaaaaaa acaaa
//