LOCUS       CO960950                 678 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15a05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO960950
VERSION     CO960950.1  GI:51334134
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 682
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgcgcatatc gcatttcatt tgccgcacat caaagttctc tctctctctc tcagcgcgca
       61 tttgttgaaa acgaatagat ccgaaccgag catgggcgtg aacacggagc tgctccaaaa
      121 acaggtgcag gcgctaaagc taagcaaaga gactggtggc gactactata aagagttgtt
      181 cgcccaacac tcggatgtgt gcgacgcgta cgggggcatt gacccggacg cggtgcccaa
      241 atcggccagg tacatcatga tggccaacaa cgaactgcag gccttcatgc aattgccgaa
      301 gaacttcaac gaggaacgcg actggcgctc ggccctgtcc aacttcaagg agcactacag
      361 cgacagcgac gtcccgctca aactctttaa caaaacaaag gagccgttct tggcgttgct
      421 gcaaaagcac gcggggggaa tgagtgctga gcagaaaaag ggctgggagg agctgctgga
      481 caaggcttac gccgacatga aaaagtacgg atggatctga gtggaactgg agacagccga
      541 aaatgaatac aatgatatcg ctttcttgag tagtggcaaa gcaattcatc ctcggacaga
      601 ttgatttgtt caattccttg acgaataaat ttatgcgctt aaaaaaaaaa aaaaaaaaaa
      661 aacaaaaact cgagacta
//