LOCUS       CO960948                 712 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe15a02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:UBC2_CAEEL P35129 UBIQUITIN-CONJUGATING ENZYME
            E2-17 KD, mRNA sequence.
ACCESSION   CO960948
VERSION     CO960948.1  GI:51334132
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 747
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgtctttgat taaaaagaaa acagaaaggc tttgaaacga attcagaagg aatagcaaga
       61 ttagggccgc gatccgccag cccaatgcag tgccggacca gttggcgacg accttttcca
      121 ttggcaggca actatcatgg gacctccaga gtctccctac caaggcggcg ttttcttctt
      181 caccatccac tttcctactg attacccgtt caaaccgccc aaagttgctt ttaccactcg
      241 catctaccat ccgaatatca acagcaacgg gagcatttgc ttggatattc ttagatctca
      301 gtggtctccc gcactgacca tctcaaaagt tctgctctcg atctgctcac ttctctgtga
      361 tccgaacccg gacgacccat tggtgccaga gattgcgcgc atctacaaga ccgatcgcga
      421 gagatacaac acacttgcac gggaatggac tcagaagtat gctatgtaac gggcacggcc
      481 gggataaagc gaaggctagt ggtggatatt tgaaatggca gatggatgtt tggatctttt
      541 tatgacgcgg caaatttgac aatccgtttt ttcatccatt tctcgactta tattaaataa
      601 aaatgaagga gagtcgggtt gccgtcgtct ttccaactca aatcctgtat tttcctctcg
      661 gctaacgtga taaaaaatat tgctcgtaaa aaaaaaaaaa aaaaaaaaaa aa
//