LOCUS       CO898030                 493 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe10h03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YN1I_CAEEL P91375 HYPOTHETICAL 12.1 KD PROTEIN
            K11H12.1 IN CHROMOSOME IV. [1], mRNA sequence.
ACCESSION   CO898030
VERSION     CO898030.1  GI:51237820
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 511
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggatgaactc cattatatca gcaattgctc gcaggacaat gatgaactcg tcggaaagca
       61 gtgcagtggg accagttggc gcagctttaa gacagaaaat agccgagttt ttcaagccaa
      121 tccacttgga ggtcgaatgc gagagtcgcc atcacaatgt cccgaaaggg gcggaaaagc
      181 acttccgggt acaaattgtg tccgacaaat ttgagggact tacccaatta cagcgccagc
      241 gaatggtgaa caaactgttg gccgaggaac tgcagagtca aatccacgcg ctaaggatag
      301 aggccaagaa gccttcggag tggactggcc aagcacaagt ggccgctccg ccatgtttgg
      361 gcggcggaaa aacgttggcc aactgatgag ccatttctat ctatatcttg tagtattctt
      421 cttcatttca cattttctat ctctagacac taaataaaaa ttttaaaatg gaaaaaaaaa
      481 aaaaaaaaac aaa
//