LOCUS       CO898027                 580 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe10g07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q21193 Q21193 SIMILAR TO PROFILIN. [1], mRNA
            sequence.
ACCESSION   CO898027
VERSION     CO898027.1  GI:51237817
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 588
            Seq primer: SL1 primer
            High quality sequence stop: 561.
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ataatgtcgt ggccagatct tgtcaacaca atcttatcgg caccgggttc gtgtccaaag
       61 ccgctatttg cggtcttgat ggaagcattt ggggaaaatc tgataatttc aatttggaac
      121 aatccgaggt gaatgctgct atgaatggcc ttaaaaattc ggatgtggcg cttgcaaacg
      181 gattgaaatt cgagggcgaa aaatatttcg tgctccaggc tgatgatgaa agaataatcg
      241 acaagaaagc tgcaaacgga ttcttcattt acaaaacggg caaagcattc atcatttcga
      301 tttatgaggg aggagttcag ccagaaatgt gtagcaagac cacaggatct ttggctgact
      361 attttcgaag catcaattac tgaatgggcg gcgagcatct tttgcacgaa tgaagaagac
      421 aaaaccaaat tcgaaatgtg tccacactaa attaatgcat aaccttctac cctagtactt
      481 aagtaagtat taattaagca cacacactac attaattgca cacacttctc aatgccatat
      541 aataaaaact gtttgctctc anaaaaaaaa aaaaaaaaaa
//