LOCUS       CO898024                 496 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe10f10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:KE2_HUMAN O15212 PROTEIN KE2, mRNA sequence.
ACCESSION   CO898024
VERSION     CO898024.1  GI:51237814
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 524
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tggaagacta gaggccaatt cgatgcggat atcgaggggt tacgacatct ggaacgagag
       61 aggggcaggt gcgtatcgaa caggcggcag ttggaaagcc aactaaccga gaacacaatg
      121 gtgaaggagg agttggatcg gctggagtcg ggggccaatg tgttcaagct gattgggcca
      181 gtgctcatca aacaggatct ggacgaggcg aagcaaaacg tgcacaaacg catcgactat
      241 atcaagacgg aaattgaacg gatggaatcc atgttggcag aatcgacgaa cactatggag
      301 agtcagaagg cctcggtaga acaaagcaga gaccaactga aacaagcgct tgcagctaca
      361 acagccgtat cccccgggca gtgatttcga tgctggtgga taaaagaagc acttttgttc
      421 actcgcagcc tgctcatgct tttgtttgac acaacagttt cgctcctttt aataaagcaa
      481 tgaacggaaa aaaaaa
//