LOCUS       CO898023                 454 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe10f09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O61861 O61861 M01B12.3 PROTEIN. [1], mRNA
            sequence.
ACCESSION   CO898023
VERSION     CO898023.1  GI:51237813
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 438.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgtgcttact gacacggctt atcgcaaact tgacgtagac gcatttgatc cggacaagtt
       61 tgatgatggc gacgaccaaa ctgaagaaac tccggcccga ctagggccag acgagagcca
      121 agtccaccaa ctgctgcaat caaatcgcaa tgtggacgcc ctgaaggctg cgcttgccaa
      181 tcccccactg gaaacgaaga ataaggctct tcgggacaaa gcgaccgctt tggttacgcg
      241 ggtgttgacc agctttaaga atgcagagat gggggcagca gtaaaggcac tctccgctga
      301 cgaggtggat ctgctgatga agtacatcta caagggcatg gagttgcttc cggatggaca
      361 aacttgcgcc tcactactcg catggcatgc acaggccttt caaatttgcg gacatggcgg
      421 cattgtacta gtttatacgg cacgaaatgg actt
//