LOCUS       CO898021                 463 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe10f07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:VATH_CAEEL Q20591 PROBABLE VACUOLAR ATP SYNTHASE
            SUBUNIT H, mRNA sequence.
ACCESSION   CO898021
VERSION     CO898021.1  GI:51237811
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 473
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tctgcttgga tcccgtggct aattgtgact ttgttttggg ctgttgtcgg aactggagga
       61 ccatttcttg ttcccagtgg tgcaaacagg ggcatcgtgc agactatgat tgtgctgaca
      121 gccgtttgtt gctacatgtt ttggctgctc gtcttccttc accaactcaa tccattgatc
      181 ggaccccaaa taccggtgaa gacaatcatg tggattcatg agcaatgggg agatgccaat
      241 aaagttgcac cataaataca cggatggact gcgacatcaa atcatcgacc acgaagccga
      301 tatatactaa tccacttcga attctgctta tctaaagaat cgacgacttg aaatgtcaaa
      361 gataaatact atcacaatta gaactattca ttaatgcact ataggtataa actatggacc
      421 ctgaaaaaaa aaaaaaaaaa aaaaaaaaaa aaacaaaaag aaa
//