LOCUS       CO898013                 599 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe10e09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:P90860 P90860 F36A2.7 PROTEIN. [1], mRNA sequence.
ACCESSION   CO898013
VERSION     CO898013.1  GI:51237803
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cttattttta ctcccatatt tgtacttctg gcagagctcg aacttcgaaa tgatcccaat
       61 ttctaaattg gcttcaccac tccacccagc ctcttcgggt gccattctcc gtgttttcgc
      121 gattcgaaaa tttgccagtg ctgttttgga acggccgcca aaggctaaga tccaactgaa
      181 accaccacca ggcgagaagg gctacatgaa ctatgaacgt gacttttcac gcgaatcacg
      241 ttatacaaag ccacataaga agaccgacac atttataaga tttgtaacat ttaacgctcg
      301 ccatcacatg gagctctggc cgatcgtatt tctctgcggt gtctcttgtt gcctagtcgt
      361 gggcatagcc tacataagtt ttgaaaaagt ggaagtctgg cttgacagaa gcaccaagac
      421 cccaccatgg gattgggcgc gcattcgtga caaatactgg agaatgcaaa cagtattttt
      481 cgatccactt ggcgggaccc accagcgtat agaaatcatg gagaagctac aggatgagat
      541 gttggaggcg gccaagcagc gtggaacccg cgatgccggt ggctacattg ttactaact
//