LOCUS       CO898006                 560 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe10d08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS3A_HUMAN P49241 40S RIBOSOMAL PROTEIN S3A. [1],
            mRNA sequence.
ACCESSION   CO898006
VERSION     CO898006.1  GI:51237796
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 394.
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgggcaagaa gggtgctaag aagaaggcgg ttgacccgtt caccagaaaa gagtggtacg
       61 acatccgtgc tccggcaatg ttccagcacc gaagtgttgg caagacactc gtgaaccgaa
      121 cccagggaac tcgcatttca agcgattttt tgaagggccg tgtctatgag gtttctttgg
      181 gtgatcttaa caacactgat gccgactttc gcaaattccg cttgatctgc gaagaagttc
      241 agggcaagaa ttgcttgacg aacttccatg gaatgacttt cactcgggac aagctctgct
      301 cgattgtcaa aaagtggcat acgcttatcg aggctaatgt ggcagtgaag accacggacg
      361 gctatctgct ccgtctgttc tgcattggct tcactatacg tagcctcaac aagatccaaa
      421 acaccagcta tgccaagact tctcaagtgc gcgccattcg ggctaaaatg gtggagataa
      481 tgctaaaaga tgtgtcctcc agccgattta cgctcatttg tctaacacct ctaattcccg
      541 gactcctcga ctctaacgtc
//