LOCUS       CO898005                 475 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe10d07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL27_CAEEL P91914 60S RIBOSOMAL PROTEIN L27, mRNA
            sequence.
ACCESSION   CO898005
VERSION     CO898005.1  GI:51237795
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 469.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ccttgctact tccattttag aaataatggg aaagattatg aagccaggtc gggtagctat
       61 tgttctcaat ggccgttttg ccggacgcaa ggcagttgtc gtaaagagct atgacgaagg
      121 ctcaaccgat cgtccttatg gacatgcgct tattgccggg ataaacaagt acccattgaa
      181 agtgacaaag cgaatgggca agaaaaaagt ggcgaagcgg atccgcatca agcccttcat
      241 aaaggtcgtc tcgctgcagc atctgctgcc gactcgttgc attttcgatg ctgatttcga
      301 caaaagcgcc gtgaacaagg agtcaattaa ggagccaaga aagaagaaga atgcgcttag
      361 caacgtgaag aaagagcttg agacgcacta caaaactggc aaaagcaagt ggttgttcac
      421 aaagctgcga ttctaaagga attttgtggt gaaaagggaa agaatgctgc tgttg
//