LOCUS       CO898001                 445 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe10c07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL27_CAEEL P91914 60S RIBOSOMAL PROTEIN L27, mRNA
            sequence.
ACCESSION   CO898001
VERSION     CO898001.1  GI:51237791
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttgctacttc cattttagaa ataatgggaa agattatgaa gccaggtcgg gtagctattg
       61 ttctcaatgg ccgttttgcc ggacgcaagg cagttgtcgt aaagagctat gacgaaggct
      121 caaccgatcg tccttatgga catgcgctta ttgccgggat aaacaagtac ccattgaaag
      181 tgacaaagcg aatgggcaag aaaaaagtgg cgaagcggat ccgcatcaag cccttcataa
      241 aggtcgtctc gctgcagcat ctgctgccga ctcgttgcat tttcgatgct gatttcgaca
      301 aaagcgccgt gaacaaggag tcaattaagg agccaagaaa gaagaagaat gcgcttagca
      361 acgtgaagaa agagcttgag acgcactaca aaactggcaa aagcaagtgg ttgttcacaa
      421 agctgcgatt ctaaaggaat tttgt
//