LOCUS       CO897989                 426 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe10b02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:DYL1_DROME Q24117 DYNEIN LIGHT CHAIN 1,
            CYTOPLASMIC. [2] TR:O96860, mRNA sequence.
ACCESSION   CO897989
VERSION     CO897989.1  GI:51237779
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 412.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 atttgagttt atctaacttc tcgctatata aaaagcatca atgggtgacc gaaaagccgt
       61 aatcaagaat gctgatatga cagaagagat gcaacaagag gcgatcgagt gcgcgactca
      121 agccctggag aagtacaaca tagagaagga tattgctgca tacatcagaa aggagtttga
      181 caaaaaattc aatccgactt ggcactgcat tgttggccga aactttggct cctatgtcac
      241 gcacgagacg aagcacttca tatacttcta cttgggccag gttgccattc tcctgttcaa
      301 gagcggctga acgtgaaata tgttcaatga agacaaatca gatcagataa actgtgtttt
      361 cgactgacgc gttctatcta cgtttaactc attaaaacct atttgttttg tctacatgga
      421 aaaaaa
//