LOCUS       CO897978                 543 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09h07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O17922 O17922 K01G5.8A PROTEIN. [3] TR:O45634
            WP:CE16198, mRNA sequence.
ACCESSION   CO897978
VERSION     CO897978.1  GI:51237768
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 597
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tctataatct aatgtcggat tcaaacaaac cggtcaaaga tcgagtgggg gaggagagga
       61 cgtgcaacaa gagccacgac ccagcgacaa cgaaagcgaa actcggaggt caaaggaatt
      121 ggtggtccgg agtgggctga aactgaagcg aggcacactg ttcaagcaga agcgccgtgt
      181 ggaccccagg accattgatc tgaccataaa gaaggagcac gccggcccaa taaagaccaa
      241 ggcggaaatt gccttcataa aaaggcaaga ggagactgcg tttgagcgtt tgtccaaacg
      301 ggcgcagacc agccacaggg agaaggtgga acgcttcaac aaacagatgg aggagctgac
      361 cgagttcaac gacatcccaa aggtttcttg gacgaagtaa tttcccttgt ggtctcaggc
      421 tatggcgaag gcggaagaag cgaaaaatgc tctattgttc attttattca attttattca
      481 caatgtgagc aaacaaacga tggtaatgac ctgatgactg gatttggata aagcattctg
      541 tat
//