LOCUS       CO897977                 472 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09h05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RLA2_CRYST O61463 60S ACIDIC RIBOSOMAL PROTEIN P2,
            mRNA sequence.
ACCESSION   CO897977
VERSION     CO897977.1  GI:51237767
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 477
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttgcgatcaa tcttctaatt cacaagcgca tcaaaatgaa gtacgtagct gcctacttgt
       61 tggctggtct cggcggaaac gcgagcccat cggccaagga cttggagaag atcctgggcg
      121 ccggcaaact cgacgtggac gtggagaacg cgaacgcggt gatcaacgcg ctcaagggca
      181 agaccgtcga ggaggtgatc gcggccggcg agaagaagct ggccagcgtg cccagcggag
      241 ctccggccgc cgcagcgcca gcggcggcag caccggccgc agctgtagct gagaagaagg
      301 aggagaaaaa ggaagagaag aaagaggaga gtgaggacga ggacatgggc ttcggactgt
      361 tcgattgaac gcgcgaccat tcgcaaaaag agcgtccact caaaccgttg ttttcgttta
      421 aaccactata aatttgttga acatgaaaaa aaaaaaaaaa aaaaaagaca aa
//