LOCUS       CO897972                 419 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09g11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:BCAT_CAEEL P54688 BRANCHED-CHAIN AMINO ACID
            AMINOTRANSFERASE, CYTOSOLIC, mRNA sequence.
ACCESSION   CO897972
VERSION     CO897972.1  GI:51237762
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 434
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cctacagccc agtttcatgc actttttcga caactgtttg tcgggctatg gcttcggtat
       61 cgggcagtcg acaagcgcat catgcagcct cggacattgt cccaactccc gaagagaaac
      121 ctgtcgacac attctatcac aaagacttga ttgttgaccg cgcttcgcca cttcaaatgc
      181 agccaaagcc gtccagcaaa gaaattttga aattcggtca catattttct gatcacatga
      241 tggaggtaga ctggagcgaa aacaacggat gggagcgtcc gagcttgtgt ccgttacaca
      301 acatcagcct tcatccagga gccaaggtgc tacattatgc gattgagctg tttgaaggca
      361 tgaaagccta cagggggatc gatgatcgga ttcggctttt ccgaccagaa aaaaacaaa
//