LOCUS       CO897965                 456 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09g03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q18502 Q18502 HYPOTHETICAL PROTEIN C36H8.3. [1],
            mRNA sequence.
ACCESSION   CO897965
VERSION     CO897965.1  GI:51237755
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 420.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gtccctcatc ggcagttcag tacactcatt ctgtttgatt ttcgccacac aaaaatgctc
       61 taccggaaaa tgatgatcgc gccctcggct gccgtcgtga tgatgtcact cctggtcagc
      121 acaggcctct gctcggctct tctctggcag cagaacgagc cgaaagacaa ggccgctcag
      181 ccggcacagg aggcagcagc agcaccgctc ttcttcttcc cggcagcaaa tgcagcggct
      241 ctggagcagt tggccgccga agaggcgcaa ctgattgcgg aggcggcggc acaacaagcg
      301 gcggccgacg aggagcaaga agcggagctt gccgctctga agccgatgcg ggcgcagacg
      361 ttcgtccgtt tcggcaaacg gtctcagatg gataatgaaa tggcggctgg catgaagccg
      421 aaaagagcac agacatttgt gcgcttcggg aaaaag
//