LOCUS       CO897959                 496 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09f08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O62337 O62337 R06C1.4 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897959
VERSION     CO897959.1  GI:51237749
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 527
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 actgtctacg gtttacgcaa agtttaatcg ctcttttaaa ttcgagaaaa tgtcttcatc
       61 tggctacagc gtttacatcg gaaacctggc ttatcaatcc acggagaacg acgtcggaaa
      121 tttgttctcc caatgtggaa ccgtcaccaa tgttcgcatt gtgtacgacc gcgagaccaa
      181 ccgtccgaag ggatttggct tctgcgagtt ccaggaccag caaggcgctc agaatgctgt
      241 aaaccagatg aacggaactg agcttcacgg tcgcgcactc cgtgtgaact ttgccaacaa
      301 gtagaggcat ggttgctgag aagccatgga atgaatgcaa acaagactca tgatcgggcc
      361 ttagaataat tccaaattta aaaccgtatt tttggattca atctttaagc cctgatcaaa
      421 cgttgttgca cctttaactt attacgcatt ctcatgagct aaaataaact gactttgtta
      481 aaaaaaaaaa aaaaaa
//