LOCUS       CO897956                 506 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09f05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:CYC1_ASCSU P92504 CYTOCHROME C, TYPE-1. [1], mRNA
            sequence.
ACCESSION   CO897956
VERSION     CO897956.1  GI:51237746
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 568
            Seq primer: SL1 primer
            High quality sequence stop: 502.
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 agctttttgc tgagactgcc aatggccgaa attcctgagg gtgactacga gaagggaaag
       61 aaagtcttca aggcccggtg tctccaatgc cacgtgatcg actccatggc cacaaagaca
      121 ggaccaaccc tgaatgggat ttggggaagg aaaagtggac aggtacccgg attcgactat
      181 tctgccgcca acaaaaataa gggtgttgtt tggaatcgcg aaacgctctt cgaatacctg
      241 ctcaatccaa agaaatatat cccggggacg aagatggttt ttgcagggct gaagaaggtc
      301 gacgaaaggg cggatctcat caagtacatc gaggttgaaa gtcagaaacc gccagcatga
      361 tgaattgacg catgagaggt tcatcgcccg tccacgacaa acaggatagt ttatagtcag
      421 tcacgtgcga gacacttggt taaaattgtt tttcacctag acattttcac actctgaggc
      481 cctcgtttct aagaaattgt tagttc
//