LOCUS       CO897955                 578 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09f04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO897955
VERSION     CO897955.1  GI:51237745
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ccccgtttgg tagctcttgc tcgcctgcct gcctgtcaca tcgtgccgaa ttcaaactga
       61 gcctccgcag caacaacgcg ccaatcttcc ccggtggtcg tgtgctcccc caaccgcagt
      121 tgcctgagtc caatccgagt gccggagatt gtgccgagtt tgagccagat aacaacgacg
      181 agaagcgaga gagccgagat gtttttcctg gacgaggaac aagagcacca atacctggag
      241 atggaggagc tgatgcgcga ggagcagcag gagctggtgc tgggcgtccg ctacggcggc
      301 aggctggacc aagagaaccg ccgcggctct gcactgctgg gcgaccccca agtcctcaca
      361 gctggcaaaa acaaaagggc tcattccctt ttgcgccgac gtgtctctct tacagacgag
      421 ttcacgtgat ttgtatgcga acgcagcagc tccaacgtga aatgcccgcg aagaaaacag
      481 aaaattgtgc ggccggcccg ctcggctgaa ttggcgaggg accgaccgac caagcgagcg
      541 ctggtcacga gcacacttat ccctccccca tccatttc
//