LOCUS       CO897953                 689 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09f01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL7A_CHICK P32429 60S RIBOSOMAL PROTEIN L7A, mRNA
            sequence.
ACCESSION   CO897953
VERSION     CO897953.1  GI:51237743
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 551.
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aagaagtcgc cgctctgccg gccgctatga agaagaccga ggcctccaag aaagtgacaa
       61 agaacacgct tttcgagaaa agaactcgca acttcgcaat aggccaggac atcattccga
      121 agcgggacct gactcgcttt gttcgatggc ccaagtacat ccttctccag cgccagctgg
      181 ccgttctgat gaagcgtctc aagatcccgc cgaccatcaa ccagttcaac tttgcattgg
      241 acaagcccaa cagtgcgcaa ctgcttaagc tgctcaacaa gtaccgaccg gagacgcgtc
      301 aggagaagaa agaacgactg cgccagcgtg cccaggaccg ggtcgagggc aaacaagaga
      361 aggtgaccaa gcgtccgccg gtggtccact tcgggatcaa ggacgtggtc aagcaagtgg
      421 agaacaagaa ggccagtctc gtggcgattg cacacgatgt tgacccgatc gagatcgtca
      481 tctgtctgcc ggccgtctgc cgcaagtttg gcgtgcccta tgtcattgtc caggggaagg
      541 ctcgccttgg acaagttgtg aaccgcaaga actgttccgc tgtgtgcctg tccgaacgtg
      601 acccggaaga acagaacgca ctgaagaact tggccgaagg ggcctccacc aacttcaacg
      661 aacggggaaa gaaaatcggc aagcactgg
//